Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker

Journal: Journal of breast cancer

Article Title: Norepinephrine/β 2 -Adrenergic Receptor Pathway Promotes the Cell Proliferation and Nerve Growth Factor Production in Triple-Negative Breast Cancer.

doi: 10.4048/jbc.2023.26.e25

Figure Lengend Snippet: Figure 5. Norepinephrine/β2-adrenergic receptor signal promotes the growth of triple-negative breast cancer cells via ERK activation. (A) Phosphorylation of ERK and CREB after NE treatment of MD-MB231 cells. After starvation, the cells were treated with 10 µM NE/ISO/Ate/Pro/ICI for 6 hours. Cell lysates were subjected to immunoblotting with antibodies against p-ERK (1:1,000), ERK (1:1,000), p-CREB (1:800), CREB (1:800), and β-actin (1:1,000). Quantitation of the western blotting results is shown in the bar graph. Data from three independent experiments are expressed as means ± SD. (B) Phosphorylation of ERK and CREB after NE treatment of BT549 cells. Data from three independent experiments are expressed as means ± SD. (C) CCK-8 assay of the MD-MB231 and BT549 cells. After starvation, MD-MB231 and BT549 cells were treated with 10 µM NE/ISO/KT5720/U0126 for 24 hours to measure the cell viability. Data are shown as means ± SD from triplicate experiments. NE = norepinephrine; ISO = isoprenaline; Ate = atenolol; Pro = propranolol; SD = standard deviation; CCK = Cell Counting Kit; β2-AR = β2-adrenergic receptor. *p < 0.01, †p < 0.001.

Article Snippet: Anti-tyrosine hydroxylase (TH) Ab (75875), anti-S100 Ab (112), and anti-β2-AR Ab (182136) were purchased from Sigma (St. Louis, USA); anti-CREB Ab (12208-1-AP), phosphorylated anti-CREB Ab (ser133), anti-ERK Ab (16443-1-AP), Phosphorylated anti-ERK Ab (T202/Y204), and anti-NGF Ab (52918) were purchased from Cell Signaling Technology (Danvers, USA); anti-beta-actin Ab was purchased from Beijing Zhongshan Jinqiao Company (Beijing, China); anti-TrkA BAS-bs (10210R) was purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China); horseradish peroxidase (HRP)-conjugated Goat Anti-Rabbit IgG (H+L) (SA00001-2) was purchased from Proteintech Group (Rosemont, USA).

Techniques: Activation Assay, Phospho-proteomics, Western Blot, Quantitation Assay, CCK-8 Assay, Standard Deviation, Cell Counting

Journal: BioMed Research International

Article Title: Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression

doi: 10.1155/2015/159015

Figure Lengend Snippet: The influence of subchronic arsenic exposure on the expression levels of 5-HT 1A receptor, PKA, TrkB, and p-Akt in the cerebral PFC. Eight weeks after exposure with 10 mg/L arsenic or drinking water alone in normal mice and in reserpine pretreated mice, the expression levels of 5-HT 1A receptor and PKA proteins (a) and the expression levels of TrkB, p-Akt (b) in the cerebral PFC of the tested mice were detected by western blot analysis. These protein expression levels were quantitated by microcomputer image device (MCID) image analysis. The lower panel shows the individual protein/ β -actin ratios while the β -actin levels were evaluated as a loading control. Each value/error bar is expressed as the mean ± SD of 8 mice. ∗ P < 0.05, ∗∗ P < 0.01, significant difference compared with normal control group (drinking water alone). # P < 0.05, significant difference from the reserpine group (Res).

Article Snippet: Rabbit polyclonal antibodies against TrkB, CREB, and PKA were purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Expressing, Western Blot, Control